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For Academics

Worm protocols

Collection of protocols and buffers for use with C. elegans.

Lexogen Quantseq library prep

Protocol to prepare 3' RNA libraries. Modified from the Lexogen protocol to use 1/4 reactions.

The numbers in gray refer to electronic repetitive pipetting.

The elutions are in larger volumes than 1/4th.

The first step could use less concentrated RNA samples, so that water is not needed. The Qiagen Plant RNA extraction kit elution volume of 60 ┬Ál should be sufficient with typical Mimulus leaf extractions.

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