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Lexogen Quantseq library prep

Protocol to prepare 3' RNA libraries. Modified from the Lexogen protocol to use 1/4 reactions.

The numbers in gray refer to electronic repetitive pipetting.

The elutions are in larger volumes than 1/4th.

The first step could use less concentrated RNA samples, so that water is not needed. The Qiagen Plant RNA extraction kit elution volume of 60 µl should be sufficient with typical Mimulus leaf extractions.

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Sex chromosome papers RSS


The genome sequence of the harbour porpoise, Phocoena phocoena (Linnaeus, 1758)
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The genome sequence of a beetle, Pycnomerus fuliginosus Erichson, 1842
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Allelic variation and duplication of the dmrt1 were associated with sex chromosome turnover in three representative Scatophagidae fish species
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