Notes on using Qubit.

1. Prepare N tubes + 2 for CS1 and CS2

2. Prepare master mix (MM)

   N tubes + 2 (CS1, CS2) + 10%

   Solution	1x (μl)
   Buffer	199
   Fluorochrome	1
   Final volume	200

   Vortex well and use within 1 hr.

3. Prepare the tubes

   CS1: 190 ul (MM) + 10 μl (CS1) CS2: 190 ul (MM) + 10 μl (CS2) Samples: 198 ul (MM) + 2 μl (DNA)

   Vortex well and centrifuge the tubes and let stand for 5 min.

4. Use Qubit

   Select 'Quant IT dsDNA HS'

   1. Put CS1 and read
   2. Put CS2 and read
   3. Keep CS2 and 'sample' > 'calculate sample concentration' > '10 μl' > 'ng/μl'
   4. Add the first sample 'read' > 'calculate sample concentration' > '2 μl' > 'ng/μl'

   Remember to remove the last stample from the machine.

   Record your usage in the log.