Fluorescent in-situ hybridisation in M. annua.
We are developing hairy root cultures in Mercurialis lineages. These are a good source of mitotic chromosomes.
We will use FISH and GISH to characterise the state of the Y chromosome for example the accumulation of transposable elements, but also to confirm the hypothesised parental species that are involved in polyploidisation in the genus. Y-specific markers could also be developed to confirm whether the different ploidy lineages use the same sex chromosomes, as well as the number of those chromosomes in the polyploids.